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2 edition of Identification and characterization of CNrasGEF and other Nedd4-interacting proteins. found in the catalog.

Identification and characterization of CNrasGEF and other Nedd4-interacting proteins.

Nam Pham

Identification and characterization of CNrasGEF and other Nedd4-interacting proteins.

by Nam Pham

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  • 25 Currently reading

Published .
Written in English


The Physical Object
Pagination339 leaves.
Number of Pages339
ID Numbers
Open LibraryOL22493649M
ISBN 100612745759

THE IDENTIFICATION OF NRAGE: A NOVEL IAP-INTERACTING PROTEIN Dissertation I hereby declare that the submitted dissertation was completed by myself and no other. I have not used any sources or materials other than those enclosed. The screen resulted in the identification of several overlapping fragments of a previously.   Despite the identification of many centrosomal and ciliary proteins, it is still not known how many of these components interact and function. We have recently characterized the human centrosome proteome in depth using a quantitative mass spectrometry-based method in combination with an antibody-based screen, identifying several novel and Author: L Jakobsen, JM Schrøder, K Vanselow, EA Nigg, E Lundberg, J Andersen.

On the contrary, the protein level of CNrasGEF decreased after NEDD overexpression but increased after NEDD downregulation (Figure 2C and 2D). These results show that NEDD indeed regulated the protein level of CNrasGEF and this regulation effect happened at the post-translational stage, suggesting the potential ubiquitinating by: Identification and characterization of essential genes in the human genome each other (both methods: P proteins that engage in more protein-.

identification and characterization: Concepts and case studies Federica Consiglio Giorgia Batelli Michael Van Oosten Subject of other classes. Functional Genomics Genome Sequence Gene Discovery In silico unknown proteins are compared with proteins stored in databases Subject of other classes. unw ind mRNA structu re and, in conjun ct ion with other tr ansl ati on factor s, it prepa re s mRNA temp late s fo r ribo so me re crui tm ent dur ing transl ati on initia tion.


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Identification and characterization of CNrasGEF and other Nedd4-interacting proteins by Nam Pham Download PDF EPUB FB2

CNrasGEF was identified in this study as a Nedd4-interacting protein, and our recent work has detected Nedd4 at the plasma membrane and in endosomes (P. Plant, F. LaFont, K. Simons and D.R., unpublished observations).

This could provide a mechanism to regulate CNrasGEF interactions with Ras at the plasma membrane and Rap1 in by: In addition, several other immune proteins in Toll/NF-κB pathway of L. vannamei, such as Lvtoll1, Lvtoll2, Lvtoll3, and Lvpelle, have also been identified and functional characterized.

These proteins have a positive effect on the expression of AMPs, which are secreted to extracellular space to defend against microorganism invaders [19] – [21].Cited by:   SPOTs membrane synthesis for ALD, ENO, and GAP proteins.

Oligopeptides (Custom Peptide Arrays) that have been immobilized on membranes (SPOTs system; Sigma-Aldrich Corp, St Louis, MO, USA) are routinely used for epitope identification, and this technology was used recently on a trichomonad immunogenic protein.

31 Thereby, Cited by: 3. The manner of ubiquitination. Ubiquitination, a post-translational modification (PTM), usually follows a highly ordered series of enzymatic reactions involving E1, E2, and E3s and targets proteins for degradation or brings about other cellular fates, such as the regulation of enzymatic activity, inflammatory signaling, endocytosis, and histone modifications and has Author: Xi Huang, Jing Chen, Wen Cao, Li Yang, Qingxiao Chen, Jingsong He, Qing Yi, He Huang, Enfan Zhang, Z.

Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes J.

Biol. Chem., (), pp. Google ScholarCited by: Identification and characterization of protein folding intermediates. The elusive nature of protein folding intermediates poses their identification and characterization as an extremely difficult task in the protein folding field.

In the case of small single domain proteins, the direct measurement of the thermodynamics and structural Cited by: 4. Identification and characterization of folding intermediates: Burst phase analysis.

Under certain circumstances proteins that exhibit a co-operative, two-state equilibrium transition, may display more complex kinetics. Such proteins are generally interpreted to fold or unfold via transiently populated by:   Nuclei are isolated and histones and other non-histone proteins are extracted using either 2 M NaCl or lithium diiodosalicylate (LIS).

The resulting ‘nuclear halos’ are digested with restriction endonucleases and incubated in the presence of end-labeled DNA probes from the region of by: J. Appl. Biol. Chem. 54(4), () Article Identification and Functional Characterization of the GALACTINOL SYNTHASE (MoGolS1) Gene in Melissa officinalis Plants.

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iNTRODUCTION 58 2. EXPERMENTAL PROCEDURES 63 3. RESULTS 68 - PHRI alternative splicing   Results. In this study, we cloned the goose TLR gene using rapid amplification of cDNA ends (RACE)and showed that geese TLR encoded a amino-acid protein, containing a signal secretion peptide, an extracellular leucine-rich repeat domain, a transmembrane domain and a Toll/interleukin-1 receptor signaling domain deduced from Cited by: 2.

Fat deposits in chickens contribute significantly to meat quality attributes such as juiciness, flavor, taste and other organoleptic properties. The quantity of fat deposited increases faster and earlier in the fast-growing chickens than in slow-growing chickens.

In this study, Affymetrix Genechip® Chicken Genome Arrays transcripts were used to compare gene Cited by:   Tapping panel dryness (TPD) is one of the most serious threats to natural rubber production. Although a great deal of effort has been made to study TPD in rubber tree, the molecular mechanisms underlying TPD remain poorly understood.

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The genes encoding lysine-rich matrix proteins (KRMPs) are one of the most highly expressed matrix genes in pearl oysters. However, the expression pattern of KRMPs is limited and the functions of them still remain unknown. In this study, we isolated and identified Cited by: 6.

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To date, the proteins ninein and Cep are Cited by: 1. Identification of single copy genes. The percentage of single copy genes identified in 29 angiosperm genomes ranged widely, from approximately 8%% (details in Table 1).The lower percentage was for Brassica rapa and Glycine max, while the higher was for Ricinus communis and Carica collinear genes were examined within each lineage, there Cited by:   On the other hand, unlabeled adenosine was able to block the binding of 10 μ m [ H]AMP to transfectants (K i = μ m) with a potency similar to that of unlabeled AMP (Fig.

5d).Whereas these results provide evidence that both AMP and adenosine bind to P2Y15, the results also demonstrate a lack of specific AMP binding sites on the. Most biological processes rely on specific interactions between proteins, but the experimental characterization of protein−protein interactions is a labor-intensive task of frequently uncertain outcome.

Computational methods based on exponentially growing genomic databases are urgently needed. It has recently been shown that coevolutionary methods are Cited by: Recently, a novel cGMP signalling cascade was uncovered in Dictyostelium, a eukaryote that diverged from the lineage leading to metazoa after plants and before both Dictyostelium and metazoa, the ancient cAMP-binding (cNB) motif of bacterial CAP has been modified and assembled with other domains into cGMP-target proteins.

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